Whole Exome Sequencing
We rely on the quality of Agilent SureSelect libraries for our whole exome sequencing service.
Whole Exome Sequencing
We offer WES sequencing service using DNBSEQ™ technology and Agilent SureSelect libraries. The generated FASTQs are delivered through the Dreamgenics server or via hard disk, depending on the total file size.
Whole Exome Sequencing (WES) includes the coding regions (exons) of more than 20,000 genes described to date. It is estimated that about 85% of the known disease-causing mutations are located in the exons, which is why its analysis is one of the genomic tools with the highest diagnostic yield, especially in complex cases.
In addition, the identification and characterization of structural alterations in the genome (CNVs, mobile elements, inversions and translocations) can contribute to the diagnosis of some genetic diseases, as well as help to establish the necessary preventive strategies and implement the most appropriate treatments for each patient.
The WES sequencing we perform at Dreamgenics includes:
- Project follow-up with a member of our team.
- Possibility of DNA extraction from different biological samples.
- Sequencing parameters adapted to the characteristics of the project.
- Quality control of samples, libraries and sequencing.
- Estimated delivery time 10-15 working days.
- Possibility to carry out subsequent bioinformatics analysis, if desired.
NGS Sequencing Service
Stages of service
01
Initial counseling
We help you choose the sequencing conditions best suited to your project
02
Receipt of samples
We receive your samples in our laboratory and perform quality control.
03
Library preparation
We carry out the automated preparation of libraries and the quality control of the product obtained.
04
NGS sequencing
We perform the sequencing according to the chosen conditions and deliver the FASTQs to you via HDD or our FTP.
Available options
For WES sequencing we use Agilent SureSelect XT HS technology and Agilent SureSelect Human All Exon V8 and Agilent SureSelect Clinical Research Exome V4 probes.
| SureSelect V8 | SureSelect CRE V4 | |
|---|---|---|
|
Target region |
Protein coding regions by RefSeq, CCDS and GENCODE |
|
|
Mitochondrial DNA |
No |
Yes |
|
Library preparation |
Automated on MGISP-100 robot (IVD) |
Automated on MGISP-100 robot (IVD) |
|
Recommended output |
>100X (10-12 Gb approx.) |
>100X (12-14 Gb approx.) |
|
Recommended sequencing |
PE150 |
PE150 |
|
Sequencing quality |
Q30 >85% with PE150 |
Q30 >85% with PE150 |
|
Platform |
DNBSEQ-G400 (IVD) |
DNBSEQ-G400 (IVD) |
|
Delivery time |
10-15 working days |
10-15 working days |
*The output depends on the experimental design of each project. Contact us and we will help you choose the sequencing conditions that best suit your needs.
Datasheet of the probes
Contact our team
Samples and shipping conditions
| SureSelect V8 | SureSelect CRE V4 | |
|---|---|---|
|
Species |
Human |
Human |
|
Sample |
DNA from blood, saliva, tissue, FFPE, etc. |
DNA from blood, saliva, tissue, FFPE, etc. |
|
Input and quality of samples |
|
|
|
Terms and conditions of shipment |
Cold 4ºC or Room temperature |
Cold 4ºC or Room temperature |
*If you wish, we can take care of the sample extraction using Qiagen and Invitrogen kits. Contact us for more information.
Animal samples
Experts in genomic data analysis
Find out how we can help you get the most out of your NGS data
We invite you to take a short video call with our team in which we will show you the features of our Genome One Reports platform and all its functionalities.
