RNA Sequencing
We offer you different options of massive sequencing for the study of gene expression.
RNA sequencing
We offer RNA sequencing service using DNBSEQ™ technology. The generated FASTQs are delivered through the Dreamgenics server or via hard disk, depending on the total file size.
Transcriptome analysis allows the quantification of gene expression levels at a specific time and under specific experimental conditions. These expression patterns are responsible for the different properties of cells and tissues, therefore, their study in different cell populations provides a better understanding of the functional elements of the genome.
The RNA-Seq sequencing we perform at Dreamgenics includes:
- Project follow-up with a member of our team.
- Possibility of RNA extraction from different biological samples.
- Sequencing parameters adapted to the characteristics of the project.
- Quality control of samples, libraries and sequencing.
- Estimated delivery time 10-15 working days.
- Possibility to carry out subsequent bioinformatics analysis, if desired.
NGS Sequencing Service
Stages of service
01
Initial counseling
We help you choose the sequencing conditions best suited to your project
02
Receipt of samples
We receive your samples in our laboratory and perform quality control.
03
Library preparation
We carry out the automated preparation of libraries and the quality control of the product obtained.
04
NGS sequencing
We perform the sequencing according to the chosen conditions and deliver the FASTQs to you via HDD or our FTP.
Available options
The sequencing parameters can be adapted to the user's preferences and to the characteristics of each experiment. Likewise, we can perform RNA extraction from different biological samples.
| Total RNA-Seq | mRNA-Seq | miRNA-Seq | |
|---|---|---|---|
|
Bookstore |
MGIEasy Fast RNA Library |
MGIEasy Fast RNA Library |
MGIEasy Small RNA Library |
|
Type of bookstore |
|
|
Conventional |
|
Library preparation |
Automated on robot MGISP-100 (IVD) |
Automated on robot MGISP-100 (IVD) |
Automated on robot MGISP-100 (IVD) |
|
Recommended output |
60 Mill. readings |
40 Mill. readings |
20 Mill. readings |
|
Recommended sequencing |
PE150 |
PE150 |
SE50 |
|
Sequencing quality |
Q30 >85% with PE150 |
Q30 >85% with PE150 |
Q30 >90% with SE50/SE100 |
|
Platform |
DNBSEQ-G400 (IVD) |
DNBSEQ-G400 (IVD) |
DNBSEQ-G400 (IVD) |
|
Delivery time |
10-15 working days |
10-15 working days |
10-15 working days |
*The output depends on the experimental design of each project. Contact us and we will help you choose the sequencing conditions that best suit your needs.
Contact our team
Samples and shipping conditions
| Total RNA-Seq | mRNA-Seq | miRNA-Seq | |
|---|---|---|---|
|
Species |
Human, animal, plant and microorganism |
Human, animal and plant |
Human, animal and plant |
|
Sample |
Total RNA from blood, saliva, tissue, FFPE, etc. |
Total RNA from blood, saliva, tissue, FFPE, etc. |
Total RNA from blood, saliva, tissue, FFPE, etc. |
|
Input and quality of samples |
|
|
≥500 ng, RIN≥7. |
|
Pretreatment |
Depletion of rRNA or rRNA + Globin mRNA |
mRNA capture |
- |
|
Terms and conditions of shipment |
Dry ice |
Dry ice |
Dry ice |
*For Total RNA-Seq sequencing, ribosomal RNA depletion is performed with the MGIEasy rRNA Depletion V1.3 kit. In the case of blood samples, ribosomal RNA and globin mRNA depletion is performed with the MGIEasy rRNA & Globin Depletion V1 kit.3. For mRNA-Seq sequencing, mRNA capture is performed with the Dynabeads™ mRNA purification kit from Invitrogen.
Experts in bioinformatics analysis
Find out how we can help you get the most out of your NGS data
We invite you to take a short video call with our team in which we will show you the features of our Genome One Reports platform and you will learn about the different RNA-Seq analyses we offer.
