RNA Sequencing
We offer you different options of massive sequencing for the study of gene expression.
NGS TECHNOLOGY
RNA sequencing
We guide you through the entire process, from experimental design and library preparation to data delivery. Our RNA-Seq sequencing service includes:
Project follow-up with a member of our team.
Possibility of RNA extraction from different biological samples.
Sequencing parameters adapted to the characteristics of the project.
Quality control of samples, libraries and sequencing.
Estimated delivery time of 10–15 business days.
Possibility to carry out subsequent bioinformatics analysis, if desired.
NGS Sequencing Service
HOW WE WORK
Expert guidance throughout the entire process
Available options
Choose the bookstore you want
The sequencing parameters can be adapted to the user's preferences and to the characteristics of each experiment. Likewise, we can perform RNA extraction from different biological samples.
| Total RNA-Seq | mRNA-Seq | miRNA-Seq | |
|---|---|---|---|
|
Bookstore |
MGIEasy Fast RNA Library |
MGIEasy Fast RNA Library |
MGIEasy Small RNA Library |
|
Type of bookstore |
|
|
Conventional |
|
Library preparation |
Automated on robot MGISP-100 (IVD) |
Automated on robot MGISP-100 (IVD) |
Automated on robot MGISP-100 (IVD) |
|
Recommended output |
60 Mill. readings |
40 Mill. readings |
20 Mill. readings |
|
Recommended sequencing |
PE150 |
PE150 |
SE50 |
|
Sequencing quality |
Q30 >85% with PE150 |
Q30 >85% with PE150 |
Q30 >90% with SE50/SE100 |
|
Platform |
DNBSEQ-G400 (IVD) |
DNBSEQ-G400 (IVD) |
DNBSEQ-G400 (IVD) |
|
Delivery time |
10-15 working days |
10-15 working days |
10-15 working days |
*The output depends on the experimental design of each project. Contact us and we will help you choose the sequencing conditions that best suit your needs.
Contact our team
technical aspects
Samples and shipping conditions
| Total RNA-Seq | mRNA-Seq | miRNA-Seq | |
|---|---|---|---|
|
Species |
Human, animal, plant and microorganism |
Human, animal and plant |
Human, animal and plant |
|
Sample |
Total RNA from blood, saliva, tissue, FFPE, etc. |
Total RNA from blood, saliva, tissue, FFPE, etc. |
Total RNA from blood, saliva, tissue, FFPE, etc. |
|
Input and quality of samples |
|
|
≥500 ng, RIN≥7. |
|
Pretreatment |
Depletion of rRNA or rRNA + Globin mRNA |
mRNA capture |
- |
|
Terms and conditions of shipment |
Dry ice |
Dry ice |
Dry ice |
*For Total RNA-Seq sequencing, ribosomal RNA depletion is performed with the MGIEasy rRNA Depletion V1.3 kit. In the case of blood samples, ribosomal RNA and globin mRNA depletion is performed with the MGIEasy rRNA & Globin Depletion V1 kit.3. For mRNA-Seq sequencing, mRNA capture is performed with the Dynabeads™ mRNA purification kit from Invitrogen.
Experts in bioinformatics analysis
Find out how we can help you get the most out of your NGS data
We invite you to take a short video call with our team in which we will show you the features of our Genome One Reports platform and you will learn about the different RNA-Seq analyses we offer.
THEY TRUST US
Centers and Institutions
Hospitals, universities, and research institutes in Europe and Latin America.