Transcriptomic analysis
The importance of gene expression in the study of different diseases.
How We Analyze Your RNA-Seq Data
Study of gene expression
Transcriptomic analyses allow for the quantification of gene expression through a bioinformatics analysis workflow structured into several key stages to ensure reliable and biologically relevant results.
Quality control of the sequences.
Alignment of reads against reference sequences.
Quantification of gene expression in the sample.
Differential gene expression analysis with p-value and with adjusted p-value.
Enrichment study of gene ontologies and pathways.
Study of isoforms generated by alternative splicing events.*
Identification of other RNAs (small RNAs and ncRNAs).*
*Where the experimental design and coverage allow.
Bioinformatics Services
RESULTS IN GENOME ONE REPORTS
Explore gene expression visually
The results of all analyses result in interactive graphs that can be explored on our Genome One Reports platform and downloaded as PDFs for use in scientific publications.
AVAILABLE OPTIONS
Choose your RNA-Seq experiment
The library and sequencing conditions depend on the starting sample and the type of RNA you want to study. We'll help you choose the best option.
mRNA-Seq & Total RNA-Seq
RecommendedCoding transcriptome and different RNA biotypes.
- DNBSEQ™-G400 Platform (IVD)
- MGIEasy Fast RNA Library
- mRNA Analysis / mRNA + RNA Biotypes
- >500 ng of total RNA with RIN > 7
- Recommended depth: 40–60 M per sample*
- Minimum 3 samples per condition
- Differential expression, ontologies and pathways
- Results in Genome One Reports
miRNA-Seq
Small regulatory RNA.
- DNBSEQ™-G400 Platform (IVD)
- MGIEasy Small RNA Library
- miRNA analysis
- >500 ng of total RNA with RIN > 7
- Recommended depth: 20 M per sample
- Minimum 3 samples per condition
- Differential expression, ontologies and pathways
- Results in Genome One Reports
*For Total RNA-Seq sequencing, ribosomal RNA depletion is performed with the MGIEasy rRNA Depletion V1.3 kit. In the case of blood samples, ribosomal RNA and globin mRNA depletion is performed with the MGIEasy rRNA & Globin Depletion V1 kit.3. For mRNA-Seq sequencing, mRNA capture is performed with the Dynabeads™ mRNA purification kit from Invitrogen.
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THEY TRUST US
Centers and Institutions
Hospitals, universities, and research institutes in Europe and Latin America.
Shall we talk about your RNA-Seq project?
We'll help you design your RNA-Seq experiment and interpret the results. Let us know what you need.